Glad to share the joy on our successful publication open access high impact journal Processes. Thank you for other co-researchers in contributing their inputs to this manuscript. The work involved the enrichment process of four chemical markers present in oil palm leaf extract as part of herbal standardization protocol. The presence of these four compounds viz orientin, isoorientin, vitexin and isovitexin as an enriched form has enhanced the biological properties particularly antioxidant radical scavenging activities. The qualitative and quantitative analysis was performed using ultra-high performance liquid chromatography tandem ultraviolet/photodiode array and mass spectrometry. An insight on the adsorption properties such as Langmuir, Freundlich, thermodynamic as well as adsorption behaviors of compounds of interest onto the adsorbents was also comprehended thus provided an additional information on the separation and purification process for this plant.
Abstract
Oil palm leaves (OPL) containing flavonoid C-glycosides are abundantly generated as oil palm byproducts. The performances of three macroporous resins with different physical and chemical properties for the enrichment of isoorientin, orientin, vitexin, and isovitexin from acid-hydrolyzed OPL (OPLAH) extract were screened. The XAD7HP resin exhibited the best sorption capacities for the targeted flavonoid C-glycosides and was thus selected for further evaluation. Static adsorption using the XAD7HP resin under optimal conditions (extract adjusted to pH 5, shaken at 298 K for 24 h) gave adsorption kinetics that fit well with a pseudo-second-order kinetic model. The adsorption of isoorientin and orientin was well described by Langmuir isotherms, while vitexin and isovitexin fit well with the Freundlich isotherms. Dynamic sorption trials using the column-packed XAD7HP resin produced 55–60-fold enrichment of isovitexin and between 11 and 25-fold enrichment of isoorientin, vitexin, and orientin using aqueous ethanol. The total flavonoid C-glycoside-enriched fractions (enriched OPLAH) with isoorientin (247.28–284.18 µg/mg), orientin (104.88–136.19 µg/mg), vitexin (1197.61–1726.11 µg/mg), and isovitexin (13.03–14.61 µg/mg) showed excellent antioxidant free radical scavenging activities compared with their crude extracts, with IC50 values of 6.90–70.63 µg/mL and 44.58–200.00 µg/mL, respectively. Hence, this rapid and efficient procedure for the preliminary enrichment of flavonoid C-glycosides by using macroporous resin may have practical value in OPL biomass waste utilization programs to produce high value-added products, particularly in the nutraceuticals, cosmeceuticals, pharmaceuticals, and fine chemicals industries.
Read full article: https://doi.org/10.3390/pr9040659
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